<div id="readability-page-1"><div> <div> The researchers identified a potential therapeutic vulnerability in pediatric high-grade gliomas , /PRNewswire/ -- A new study, led by researchers at Children's Hospital of Philadelphia (CHOP), identified tiny pieces of messenger RNA that are missing in pediatric high-grade glioma tumors but not in normal brain tissues. Preclinical research indicates that these missing RNA fragments can make difficult-to-treat tumors more responsive to immunotherapy. The findings were recently published in the journal Cell Reports. One of the biggest challenges facing cancer research is the need to find safe and effective therapies for the most aggressive types of brain tumors. Adoptive immunotherapies with CAR-T cells are promising; however, they often also target healthy cells, which share most surface proteins with cancerous cells. While this collateral damage might be tolerable in patients with certain types of blood cancer, in the brain, wiping out healthy neurons is unacceptable. This means that deep knowledge of gene expression patterns exclusive to tumor cells is critical. A potential means of discovering new therapeutic targets for brain tumors may lie in alternative splicing, a process whereby a single gene produces multiple proteins by rearranging exons, the building blocks of messenger RNA, in different combinations. Researchers suspected that splicing in glioma cells may differ from splicing in normal brain cells, which could help devise new therapeutic interventions. In this study, researchers found that prior RNA sequencing analyses of high-grade gliomas failed to account for some very short exons called &#34;microexons.&#34; Deeper analysis revealed that in glioma, many of these microexons fail to be incorporated into messenger RNAs encoding important surface proteins, including the neuronal cell adhesion molecule known as NRCAM. For normal brain cells to make close contacts known as synapses, full-length NRCAM is needed, but in pediatric high-grade gliomas, two NRCAM microexons were consistently skipped, resulting in a distinct protein structure with unknown function. When studying these microexons in more detail, the researchers found that the shortened version of NRCAM generated through microexon skipping was essential for cancer cell migration and invasion in Petri dishes and for tumor growth in a preclinical mouse model implanted with glioma cells. This makes the glioma-specific version of NRCAM an especially attractive immunotherapy target because the tumors won't be able to shut it down easily. &#34;While microexons may be small, the effects they have on the overall protein structure are quite profound,&#34; said senior study author Andrei Thomas-Tikhonenko, PhD, chief of the Division of Cancer Pathobiology at CHOP and Professor of in the Department of Pathology and Laboratory Medicine at the Perelman School of Medicine of the University of Pennsylvania. &#34;Because the skipping of NRCAM microexons profoundly changes protein conformation, we were able to develop a mouse monoclonal antibody against the glioma-specific version of NRCAM. When mixed with glioma cells, the antibody worked like a highlighter, &#34;painting&#34; glioma cells and marking them for killing by T cells armed with an immune receptor for mouse antibodies.&#34; &#34;In addition to developing these immune receptors clinically, we are actively using our proof-of-principle experiments to design traditional CAR T cell-based immunotherapeutics that selectively target glioma cells,&#34; said first study author Priyanka Sehgal, PhD, a research scientist in the Thomas-Tikhonenko laboratory at CHOP. &#34;This could also change the way we find new targets in other solid tumors.&#34; The next steps for this work will be to expand preclinical research and identify a specific form of immunotherapy that could potentially be explored in a clinical trial. The researchers also noted that similar molecular mechanisms have been observed in other tumors such glioblastoma multiforme and cancers of neuroendocrine origin, which also could be targeted with NRCAM-directed immunotherapeutics. This study was supported mainly by the CureSearch for Children's Cancer Foundation Acceleration Initiative and also by the National Institutes of Health grants U01 CA232563, R03 CA293992, R01 HG013359, UG3 CA290451 and R01 EB026892, and NIH training grants T32 CA115299, T32 HL007150 and T32 CA009140. Additional support was provided by the National Science Foundation Graduate Research Fellowship Program, Cancer Research Society Next Generation of Scientists Award, the Children's Brain Tumor Network,the Chad Tough Foundation, and the Mildred L. Roeckle Endowed Chair in Pathology at Children's Hospital of Philadelphia. Sehgal et al, &#34;NRCAM variant defined by microexon skipping is a targetable cell surface proteoform in high-grade gliomas.&#34; Cell Rep. Online August 7, 2025. DOI: 10.1016/j.celrep.2025.116099. About Children's Hospital of Philadelphia: A non-profit, charitable organization, Children's Hospital of Philadelphia was founded in 1855 as the nation's first pediatric hospital. Through its long-standing commitment to providing exceptional patient care, training new generations of pediatric healthcare professionals, and pioneering major research initiatives, the hospital has fostered many discoveries that have benefited children worldwide. Its pediatric research program is among the largest in the country. The institution has a well-established history of providing advanced pediatric care close to home through its CHOP Care Network, which includes more than 50 primary care practices, specialty care and surgical centers, urgent care centers, and community hospital alliances throughout Pennsylvania and New Jersey. CHOP also operates the Middleman Family Pavilion and its dedicated pediatric emergency department in King of Prussia, the Behavioral Health and Crisis Center (including a 24/7 Crisis Response Center) and the Center for Advanced Behavioral Healthcare, a mental health outpatient facility. Its unique family-centered care and public service programs have brought Children's Hospital of Philadelphia recognition as a leading advocate for children and adolescents. For more information, visit https://www.chop.edu. Contact: Jennifer Lee Children's Hospital of Philadelphia (610) 800-6592 [email protected] SOURCE Children's Hospital of Philadelphia <img src="https://imageproxy.pbkrs.com/https://rt.prnewswire.com/rt.gif/query-RGF0ZUlkPTIwMjUwODE1Jk5ld3NJdGVtSWQ9REM1MjUwNSZUcmFuc21pc3Npb25fSWQ9MjAyNTA4MTUxMDQ5UFJfTkVXU19VU1BSX19fX19EQzUyNTA1?x-oss-process=image/auto-orient,1/interlace,1/resize,w_1440,h_1440/quality,q_95/format,jpg" original-src="https://imageproxy.pbkrs.com/https://rt.prnewswire.com/rt.gif/query-RGF0ZUlkPTIwMjUwODE1Jk5ld3NJdGVtSWQ9REM1MjUwNSZUcmFuc21pc3Npb25fSWQ9MjAyNTA4MTUxMDQ5UFJfTkVXU19VU1BSX19fX19EQzUyNTA1"/></div> </div></div>

Researchers at Children's Hospital of Philadelphia have discovered missing messenger RNA fragments in pediatric high-grade gliomas that could enhance immunotherapy effectiveness. The study highlights how these microexons affect protein structures, particularly the NRCAM protein, which is crucial for tumor growth and invasion. This glioma-specific NRCAM variant presents a promising target for immunotherapy, potentially leading to new treatments for aggressive brain tumors. The research aims to expand preclinical studies and explore clinical trial opportunities for targeted immunotherapy.

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10:49 ET
			
			
				Children's Hospital of Philadelphia Researchers Find that Missing Messenger RNA Fragments Could be Key to New Immunotherapy for Hard-to-Treat Tumors

10:49 ET Children's Hospital of Philadelphia Researchers Find that Missing Messenger RNA Fragments Could be Key to New Immunotherapy for Hard-to-Treat Tumors